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pgex4t1 rpb4  (Addgene inc)


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    Structured Review

    Addgene inc pgex4t1 rpb4
    Pgex4t1 Rpb4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex4t1+rpb4/pGEX4T1-Rpb4%2F7+(Plasmid+%23138484)/pm32886723-184-28-36
    Average 91 stars, based on 1 article reviews
    pgex4t1 rpb4 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90 ̊C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90°C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from IDT, and the primers were annealed by cooling from 90°C to room temperature in a water bath.

    Amplification:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90 ̊C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90°C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from IDT, and the primers were annealed by cooling from 90°C to room temperature in a water bath.

    Introduce:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90 ̊C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90°C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from IDT, and the primers were annealed by cooling from 90°C to room temperature in a water bath.

    Sequencing:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90 ̊C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90°C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from IDT, and the primers were annealed by cooling from 90°C to room temperature in a water bath.

    Clone Assay:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90 ̊C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from Integrated DNA Technologies (IDT), and the primers were annealed by cooling from 90°C to room temperature in a water bath.

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: .. Rpb7 was PCR amplified from HEK293T cDNA with primers to introduce a Shine-Delgarno sequence and a C-terminal 6x-His tag and cloned into the EcoRI and NotI sites of pGEX4T1-Rpb4 using T4 DNA ligase to generate pGEX4T1-Rpb4/7 (Addgene #138484). .. The 2x and 4x CTD repeat inserts were ordered as a pair of oligonucleotides from IDT, and the primers were annealed by cooling from 90°C to room temperature in a water bath.



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